Construct: shRNA TRCN0000006519

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003998.2-497s1c1
DNA Barcode:
GCCTGAACAAATGTTTCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NFKB1 (4790)
Gene Description:
nuclear factor kappa B subunit 1
Transcript:
RefSeq NM_003998.2 (NON-CURRENT)
Match location:
Position 497 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000006519)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGAACAAATGTTTCATTT
Hairpin Sequence:
5'-CCGG-GCCTGAACAAATGTTTCATTT-CTCGAG-AAATGAAACATTTGTTCAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGAACAAATGTTTCATTTCTCGAGAAATGAAACATTTGTTCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGAACAAATGTTTCATTTCTCGAGAAATGAAACATTTGTTCAGGC-3'

Other clones with same target sequence:

  1. TRCN0000277725