Construct: shRNA TRCN0000006543

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003939.2-1532s1c1
DNA Barcode:
GCGTTGTATTCGATTTGATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BTRC (8945)
Gene Description:
beta-transducin repeat containing E3 ubiquitin protein ligase
Transcript:
RefSeq NM_003939.2 (NON-CURRENT)
Match location:
Position 1532 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000006543)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTTGTATTCGATTTGATAA
Hairpin Sequence:
5'-CCGG-GCGTTGTATTCGATTTGATAA-CTCGAG-TTATCAAATCGAATACAACGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTTGTATTCGATTTGATAACTCGAGTTATCAAATCGAATACAACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTTGTATTCGATTTGATAACTCGAGTTATCAAATCGAATACAACGC-3'

Other clones with same target sequence:

  1. TRCN0000314899