Construct: shRNA TRCN0000006557

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018315.2-1596s1c1
DNA Barcode:
CCAGTCGTTAACAAGTGGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FBXW7 (55294)
Gene Description:
F-box and WD repeat domain containing 7
Transcript:
RefSeq NM_018315.2 (NON-CURRENT)
Match location:
Position 1596 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000006557)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTCGTTAACAAGTGGAAT
Hairpin Sequence:
5'-CCGG-CCAGTCGTTAACAAGTGGAAT-CTCGAG-ATTCCACTTGTTAACGACTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTCGTTAACAAGTGGAATCTCGAGATTCCACTTGTTAACGACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTCGTTAACAAGTGGAATCTCGAGATTCCACTTGTTAACGACTGG-3'

Other clones with same target sequence:

(none)