Construct: shRNA TRCN0000006654

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000014.3-4377s1c1
DNA Barcode:
CCATAGTGAAAGTCTATGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
A2M (2)
Gene Description:
alpha-2-macroglobulin
Transcript:
RefSeq NM_000014.3 (NON-CURRENT)
Match location:
Position 4377 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000006654)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATAGTGAAAGTCTATGATT
Hairpin Sequence:
5'-CCGG-CCATAGTGAAAGTCTATGATT-CTCGAG-AATCATAGACTTTCACTATGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATAGTGAAAGTCTATGATTCTCGAGAATCATAGACTTTCACTATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATAGTGAAAGTCTATGATTCTCGAGAATCATAGACTTTCACTATGG-3'

Other clones with same target sequence:

(none)