Construct: shRNA TRCN0000006680

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001132.1-636s1c1
DNA Barcode:
ACAAACAATCTGTGCGTGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AFG3L1P (172)
Gene Description:
AFG3 like matrix AAA peptidase subunit 1, pseudogene
Transcript:
RefSeq NM_001132.1 (NON-CURRENT)
Match location:
Position 636 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000006680)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAAACAATCTGTGCGTGTTA
Hairpin Sequence:
5'-CCGG-ACAAACAATCTGTGCGTGTTA-CTCGAG-TAACACGCACAGATTGTTTGT-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAAACAATCTGTGCGTGTTACTCGAGTAACACGCACAGATTGTTTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAAACAATCTGTGCGTGTTACTCGAGTAACACGCACAGATTGTTTGT-3'

Other clones with same target sequence:

(none)