Construct: shRNA TRCN0000006682

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000029.1-1947s1c1
DNA Barcode:
CCTCCGTGTAGTGTCTGTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AGT (183)
Gene Description:
angiotensinogen
Transcript:
RefSeq NM_000029.1 (NON-CURRENT)
Match location:
Position 1947 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000006682)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCGTGTAGTGTCTGTAAT
Hairpin Sequence:
5'-CCGG-CCTCCGTGTAGTGTCTGTAAT-CTCGAG-ATTACAGACACTACACGGAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCGTGTAGTGTCTGTAATCTCGAGATTACAGACACTACACGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCGTGTAGTGTCTGTAATCTCGAGATTACAGACACTACACGGAGG-3'

Other clones with same target sequence:

(none)