Construct: shRNA TRCN0000006702

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001642.1-393s1c1
DNA Barcode:
CCAGCGGGTTAGTATTGACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
APLP2 (334)
Gene Description:
amyloid beta precursor like protein 2
Transcript:
RefSeq NM_001642.1 (NON-CURRENT)
Match location:
Position 393 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000006702)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCGGGTTAGTATTGACAA
Hairpin Sequence:
5'-CCGG-CCAGCGGGTTAGTATTGACAA-CTCGAG-TTGTCAATACTAACCCGCTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCGGGTTAGTATTGACAACTCGAGTTGTCAATACTAACCCGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCGGGTTAGTATTGACAACTCGAGTTGTCAATACTAACCCGCTGG-3'

Other clones with same target sequence:

(none)