Construct: shRNA TRCN0000006724

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033028.2-254s1c1
DNA Barcode:
GCATTGATATTTCGCCTAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BBS4 (585)
Gene Description:
Bardet-Biedl syndrome 4
Transcript:
RefSeq NM_033028.2 (NON-CURRENT)
Match location:
Position 254 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000006724)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATTGATATTTCGCCTAGAA
Hairpin Sequence:
5'-CCGG-GCATTGATATTTCGCCTAGAA-CTCGAG-TTCTAGGCGAAATATCAATGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATTGATATTTCGCCTAGAACTCGAGTTCTAGGCGAAATATCAATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATTGATATTTCGCCTAGAACTCGAGTTCTAGGCGAAATATCAATGC-3'

Other clones with same target sequence:

(none)