Construct: shRNA TRCN0000006872

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002718.3-3363s1c1
DNA Barcode:
GCAGGATTATTGAAAGGATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPP2R3A (5523)
Gene Description:
protein phosphatase 2 regulatory subunit B''alpha
Transcript:
RefSeq NM_002718.3 (NON-CURRENT)
Match location:
Position 3363 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000006872)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGATTATTGAAAGGATAT
Hairpin Sequence:
5'-CCGG-GCAGGATTATTGAAAGGATAT-CTCGAG-ATATCCTTTCAATAATCCTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGATTATTGAAAGGATATCTCGAGATATCCTTTCAATAATCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGATTATTGAAAGGATATCTCGAGATATCCTTTCAATAATCCTGC-3'

Other clones with same target sequence:

(none)