Construct: shRNA TRCN0000006892

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005401.3-3772s1c1
DNA Barcode:
CGCTCAGTACAAGTTTGTCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTPN14 (5784)
Gene Description:
protein tyrosine phosphatase non-receptor type 14
Transcript:
RefSeq NM_005401.3 (NON-CURRENT)
Match location:
Position 3772 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000006892)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTCAGTACAAGTTTGTCTA
Hairpin Sequence:
5'-CCGG-CGCTCAGTACAAGTTTGTCTA-CTCGAG-TAGACAAACTTGTACTGAGCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTCAGTACAAGTTTGTCTACTCGAGTAGACAAACTTGTACTGAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTCAGTACAAGTTTGTCTACTCGAGTAGACAAACTTGTACTGAGCG-3'

Other clones with same target sequence:

(none)