Construct: shRNA TRCN0000006905

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006305.2-854s1c1
DNA Barcode:
CCTGAAGATGAGGGAGAAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANP32A (8125)
Gene Description:
acidic nuclear phosphoprotein 32 family member A
Transcript:
RefSeq NM_006305.2 (NON-CURRENT)
Match location:
Position 854 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000006905)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAAGATGAGGGAGAAGAT
Hairpin Sequence:
5'-CCGG-CCTGAAGATGAGGGAGAAGAT-CTCGAG-ATCTTCTCCCTCATCTTCAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAAGATGAGGGAGAAGATCTCGAGATCTTCTCCCTCATCTTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAAGATGAGGGAGAAGATCTCGAGATCTTCTCCCTCATCTTCAGG-3'

Other clones with same target sequence:

  1. TRCN0000280101