Construct: shRNA TRCN0000006959

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_174907.1-1173s1c1
DNA Barcode:
GCCCTGTAAGTAGTAGTTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPP4R2 (151987)
Gene Description:
protein phosphatase 4 regulatory subunit 2
Transcript:
RefSeq NM_174907.1 (NON-CURRENT)
Match location:
Position 1173 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000006959)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTGTAAGTAGTAGTTCTT
Hairpin Sequence:
5'-CCGG-GCCCTGTAAGTAGTAGTTCTT-CTCGAG-AAGAACTACTACTTACAGGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTGTAAGTAGTAGTTCTTCTCGAGAAGAACTACTACTTACAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTGTAAGTAGTAGTTCTTCTCGAGAAGAACTACTACTTACAGGGC-3'

Other clones with same target sequence:

(none)