Construct: shRNA TRCN0000006989

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015690.2-3054s1c1
DNA Barcode:
CCAGCATGGAAGTATCCTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STK36 (27148)
Gene Description:
serine/threonine kinase 36
Transcript:
RefSeq NM_015690.2 (NON-CURRENT)
Match location:
Position 3054 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000006989)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCATGGAAGTATCCTCAT
Hairpin Sequence:
5'-CCGG-CCAGCATGGAAGTATCCTCAT-CTCGAG-ATGAGGATACTTCCATGCTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCATGGAAGTATCCTCATCTCGAGATGAGGATACTTCCATGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCATGGAAGTATCCTCATCTCGAGATGAGGATACTTCCATGCTGG-3'

Other clones with same target sequence:

(none)