Construct: shRNA TRCN0000006995

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024011.1-1505s1c1
DNA Barcode:
CATCCCAACATTGTCACCGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDC2L2 (985)
Gene Description:
cell division cycle 2-like 2 (PITSLRE proteins)
Transcript:
RefSeq NM_024011.1 (NON-CURRENT)
Match location:
Position 1505 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000006995)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCCCAACATTGTCACCGTT
Hairpin Sequence:
5'-CCGG-CATCCCAACATTGTCACCGTT-CTCGAG-AACGGTGACAATGTTGGGATG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCCCAACATTGTCACCGTTCTCGAGAACGGTGACAATGTTGGGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCCCAACATTGTCACCGTTCTCGAGAACGGTGACAATGTTGGGATG-3'

Other clones with same target sequence:

(none)