Construct: shRNA TRCN0000007012

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000933.2-3147s1c1
DNA Barcode:
CGCTGACATCAGATCACAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PLCB4 (5332)
Gene Description:
phospholipase C beta 4
Transcript:
RefSeq NM_000933.2 (NON-CURRENT)
Match location:
Position 3147 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000007012)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTGACATCAGATCACAAAT
Hairpin Sequence:
5'-CCGG-CGCTGACATCAGATCACAAAT-CTCGAG-ATTTGTGATCTGATGTCAGCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTGACATCAGATCACAAATCTCGAGATTTGTGATCTGATGTCAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTGACATCAGATCACAAATCTCGAGATTTGTGATCTGATGTCAGCG-3'

Other clones with same target sequence:

  1. TRCN0000338260