Construct: shRNA TRCN0000007050

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153335.3-911s1c1
DNA Barcode:
CAGCAGAATCTCCAGGGTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STRADA (92335)
Gene Description:
STE20 related adaptor alpha
Transcript:
RefSeq NM_153335.3 (NON-CURRENT)
Match location:
Position 911 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000007050)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCAGAATCTCCAGGGTTAT
Hairpin Sequence:
5'-CCGG-CAGCAGAATCTCCAGGGTTAT-CTCGAG-ATAACCCTGGAGATTCTGCTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCAGAATCTCCAGGGTTATCTCGAGATAACCCTGGAGATTCTGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCAGAATCTCCAGGGTTATCTCGAGATAACCCTGGAGATTCTGCTG-3'

Other clones with same target sequence:

(none)