Construct: shRNA TRCN0000007141

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004145.2-3471s1c1
DNA Barcode:
GCACGTCAAGTTCCAGAACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MYO9B (4650)
Gene Description:
myosin IXB
Transcript:
RefSeq NM_004145.2 (NON-CURRENT)
Match location:
Position 3471 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000007141)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACGTCAAGTTCCAGAACAA
Hairpin Sequence:
5'-CCGG-GCACGTCAAGTTCCAGAACAA-CTCGAG-TTGTTCTGGAACTTGACGTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACGTCAAGTTCCAGAACAACTCGAGTTGTTCTGGAACTTGACGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACGTCAAGTTCCAGAACAACTCGAGTTGTTCTGGAACTTGACGTGC-3'

Other clones with same target sequence:

  1. TRCN0000273294