Construct: shRNA TRCN0000007151
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_017988.2-1131s1c1
- DNA Barcode:
- GCCCTCAGAAACCCAAAGTTA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SCYL2 (55681)
- Gene Description:
- SCY1 like pseudokinase 2
- Transcript:
- RefSeq NM_017988.2 (NON-CURRENT)
- Match location:
- Position 1131 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000007151)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCCCTCAGAAACCCAAAGTTA
- Hairpin Sequence:
-
5'-CCGG-GCCCTCAGAAACCCAAAGTTA-CTCGAG-TAACTTTGGGTTTCTGAGGGC-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCCTCAGAAACCCAAAGTTACTCGAGTAACTTTGGGTTTCTGAGGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCCTCAGAAACCCAAAGTTACTCGAGTAACTTTGGGTTTCTGAGGGC-3'
Other clones with same target sequence:
-
TRCN0000320789
