Construct: shRNA TRCN0000007190

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002954.3-284s1c1
DNA Barcode:
GAAGTCTTACACCACTCCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPS27A (6233)
Gene Description:
ribosomal protein S27a
Transcript:
RefSeq NM_002954.3 (NON-CURRENT)
Match location:
Position 284 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000007190)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGTCTTACACCACTCCCAA
Hairpin Sequence:
5'-CCGG-GAAGTCTTACACCACTCCCAA-CTCGAG-TTGGGAGTGGTGTAAGACTTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGTCTTACACCACTCCCAACTCGAGTTGGGAGTGGTGTAAGACTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGTCTTACACCACTCCCAACTCGAGTTGGGAGTGGTGTAAGACTTC-3'

Other clones with same target sequence:

(none)