Construct: shRNA TRCN0000007242

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003905.2-1638s1c1
DNA Barcode:
GCATGTCACAAACTTCAGCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NAE1 (8883)
Gene Description:
NEDD8 activating enzyme E1 subunit 1
Transcript:
RefSeq NM_003905.2 (NON-CURRENT)
Match location:
Position 1638 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000007242)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGTCACAAACTTCAGCAA
Hairpin Sequence:
5'-CCGG-GCATGTCACAAACTTCAGCAA-CTCGAG-TTGCTGAAGTTTGTGACATGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGTCACAAACTTCAGCAACTCGAGTTGCTGAAGTTTGTGACATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGTCACAAACTTCAGCAACTCGAGTTGCTGAAGTTTGTGACATGC-3'

Other clones with same target sequence:

  1. TRCN0000293361