Construct: shRNA TRCN0000007245

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003922.1-8440s1c1
DNA Barcode:
GCCCAGAATATCACTGTCCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HERC1 (8925)
Gene Description:
HECT and RLD domain containing E3 ubiquitin protein ligase family member 1
Transcript:
RefSeq NM_003922.1 (NON-CURRENT)
Match location:
Position 8440 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000007245)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAGAATATCACTGTCCTT
Hairpin Sequence:
5'-CCGG-GCCCAGAATATCACTGTCCTT-CTCGAG-AAGGACAGTGATATTCTGGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAGAATATCACTGTCCTTCTCGAGAAGGACAGTGATATTCTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAGAATATCACTGTCCTTCTCGAGAAGGACAGTGATATTCTGGGC-3'

Other clones with same target sequence:

(none)