Construct: shRNA TRCN0000007349

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004620.2-1124s1c1
DNA Barcode:
CGGAATTTCCAGGAAACTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRAF6 (7189)
Gene Description:
TNF receptor associated factor 6
Transcript:
RefSeq NM_004620.2 (NON-CURRENT)
Match location:
Position 1124 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000007349)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAATTTCCAGGAAACTATT
Hairpin Sequence:
5'-CCGG-CGGAATTTCCAGGAAACTATT-CTCGAG-AATAGTTTCCTGGAAATTCCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAATTTCCAGGAAACTATTCTCGAGAATAGTTTCCTGGAAATTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAATTTCCAGGAAACTATTCTCGAGAATAGTTTCCTGGAAATTCCG-3'

Other clones with same target sequence:

(none)