Construct: shRNA TRCN0000007402

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004840.1-657s1c1
DNA Barcode:
CCAGTAATTATGTCCGTGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARHGEF6 (9459)
Gene Description:
Rac/Cdc42 guanine nucleotide exchange factor 6
Transcript:
RefSeq NM_004840.1 (NON-CURRENT)
Match location:
Position 657 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000007402)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTAATTATGTCCGTGAAA
Hairpin Sequence:
5'-CCGG-CCAGTAATTATGTCCGTGAAA-CTCGAG-TTTCACGGACATAATTACTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTAATTATGTCCGTGAAACTCGAGTTTCACGGACATAATTACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTAATTATGTCCGTGAAACTCGAGTTTCACGGACATAATTACTGG-3'

Other clones with same target sequence:

(none)