Construct: shRNA TRCN0000007463

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005419.2-1477s1c1
DNA Barcode:
GCTTCAGTTCTCTGGTTCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STAT2 (6773)
Gene Description:
signal transducer and activator of transcription 2
Transcript:
RefSeq NM_005419.2 (NON-CURRENT)
Match location:
Position 1477 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000007463)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCAGTTCTCTGGTTCAAT
Hairpin Sequence:
5'-CCGG-GCTTCAGTTCTCTGGTTCAAT-CTCGAG-ATTGAACCAGAGAACTGAAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCAGTTCTCTGGTTCAATCTCGAGATTGAACCAGAGAACTGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCAGTTCTCTGGTTCAATCTCGAGATTGAACCAGAGAACTGAAGC-3'

Other clones with same target sequence:

(none)