Construct: shRNA TRCN0000007464
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_005419.2-1724s1c1
- DNA Barcode:
- GCAAGTTACCATTCTGGACAT
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- STAT2 (6773)
- Gene Description:
- signal transducer and activator of transcription 2
- Transcript:
- RefSeq NM_005419.2 (NON-CURRENT)
- Match location:
- Position 1724 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this shRNA:
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Sequence Information
- Target Sequence:
- GCAAGTTACCATTCTGGACAT
- Hairpin Sequence:
- 5'-CCGG-GCAAGTTACCATTCTGGACAT-CTCGAG-ATGTCCAGAATGGTAACTTGC-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCAAGTTACCATTCTGGACATCTCGAGATGTCCAGAATGGTAACTTGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCAAGTTACCATTCTGGACATCTCGAGATGTCCAGAATGGTAACTTGC-3'