Construct: shRNA TRCN0000007501

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005744.2-832s1c1
DNA Barcode:
GCCAGATGAATACAAGGTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARIH1 (25820)
Gene Description:
ariadne RBR E3 ubiquitin protein ligase 1
Transcript:
RefSeq NM_005744.2 (NON-CURRENT)
Match location:
Position 832 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000007501)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGATGAATACAAGGTCAT
Hairpin Sequence:
5'-CCGG-GCCAGATGAATACAAGGTCAT-CTCGAG-ATGACCTTGTATTCATCTGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGATGAATACAAGGTCATCTCGAGATGACCTTGTATTCATCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGATGAATACAAGGTCATCTCGAGATGACCTTGTATTCATCTGGC-3'

Other clones with same target sequence:

(none)