Construct: shRNA TRCN0000007537

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006002.3-396s1c1
DNA Barcode:
CCTGGAGGAATCTGTGTCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UCHL3 (7347)
Gene Description:
ubiquitin C-terminal hydrolase L3
Transcript:
RefSeq NM_006002.3 (NON-CURRENT)
Match location:
Position 396 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000007537)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGAGGAATCTGTGTCAAT
Hairpin Sequence:
5'-CCGG-CCTGGAGGAATCTGTGTCAAT-CTCGAG-ATTGACACAGATTCCTCCAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGAGGAATCTGTGTCAATCTCGAGATTGACACAGATTCCTCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGAGGAATCTGTGTCAATCTCGAGATTGACACAGATTCCTCCAGG-3'

Other clones with same target sequence:

  1. TRCN0000320754