Construct: shRNA TRCN0000007545

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006048.2-5289s1c1
DNA Barcode:
CCCTACTTAACATACTTGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UBE4B (10277)
Gene Description:
ubiquitination factor E4B
Transcript:
RefSeq NM_006048.2 (NON-CURRENT)
Match location:
Position 5289 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000007545)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTACTTAACATACTTGAAT
Hairpin Sequence:
5'-CCGG-CCCTACTTAACATACTTGAAT-CTCGAG-ATTCAAGTATGTTAAGTAGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTACTTAACATACTTGAATCTCGAGATTCAAGTATGTTAAGTAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTACTTAACATACTTGAATCTCGAGATTCAAGTATGTTAAGTAGGG-3'

Other clones with same target sequence:

(none)