Construct: shRNA TRCN0000007580

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006362.3-2013s1c1
DNA Barcode:
CCCTGTAAATAGTCCTTGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NXF1 (10482)
Gene Description:
nuclear RNA export factor 1
Transcript:
RefSeq NM_006362.3 (NON-CURRENT)
Match location:
Position 2013 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000007580)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGTAAATAGTCCTTGGAT
Hairpin Sequence:
5'-CCGG-CCCTGTAAATAGTCCTTGGAT-CTCGAG-ATCCAAGGACTATTTACAGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGTAAATAGTCCTTGGATCTCGAGATCCAAGGACTATTTACAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGTAAATAGTCCTTGGATCTCGAGATCCAAGGACTATTTACAGGG-3'

Other clones with same target sequence:

(none)