Construct: shRNA TRCN0000007586

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006395.1-1520s1c1
DNA Barcode:
GCTTTGGGATTTGACACATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATG7 (10533)
Gene Description:
autophagy related 7
Transcript:
RefSeq NM_006395.1 (NON-CURRENT)
Match location:
Position 1520 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000007586)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTGGGATTTGACACATTT
Hairpin Sequence:
5'-CCGG-GCTTTGGGATTTGACACATTT-CTCGAG-AAATGTGTCAAATCCCAAAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTGGGATTTGACACATTTCTCGAGAAATGTGTCAAATCCCAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTGGGATTTGACACATTTCTCGAGAAATGTGTCAAATCCCAAAGC-3'

Other clones with same target sequence:

(none)