Construct: shRNA TRCN0000007606

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006537.1-299s1c1
DNA Barcode:
CCAACCATAAGAAATCAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
USP3 (9960)
Gene Description:
ubiquitin specific peptidase 3
Transcript:
RefSeq NM_006537.1 (NON-CURRENT)
Match location:
Position 299 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000007606)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAACCATAAGAAATCAGAAA
Hairpin Sequence:
5'-CCGG-CCAACCATAAGAAATCAGAAA-CTCGAG-TTTCTGATTTCTTATGGTTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAACCATAAGAAATCAGAAACTCGAGTTTCTGATTTCTTATGGTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAACCATAAGAAATCAGAAACTCGAGTTTCTGATTTCTTATGGTTGG-3'

Other clones with same target sequence:

  1. TRCN0000320694