Construct: shRNA TRCN0000007614

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006704.2-1070s1c1
DNA Barcode:
GCTCTCATCGTATTGTGTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SUGT1 (10910)
Gene Description:
SGT1 homolog, MIS12 kinetochore complex assembly cochaperone
Transcript:
RefSeq NM_006704.2 (NON-CURRENT)
Match location:
Position 1070 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000007614)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTCATCGTATTGTGTATA
Hairpin Sequence:
5'-CCGG-GCTCTCATCGTATTGTGTATA-CTCGAG-TATACACAATACGATGAGAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTCATCGTATTGTGTATACTCGAGTATACACAATACGATGAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTCATCGTATTGTGTATACTCGAGTATACACAATACGATGAGAGC-3'

Other clones with same target sequence:

(none)