Construct: shRNA TRCN0000007653

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006937.2-273s1c1
DNA Barcode:
CCTATTGTGAACGACAGGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SUMO2 (6613)
Gene Description:
small ubiquitin like modifier 2
Transcript:
RefSeq NM_006937.2 (NON-CURRENT)
Match location:
Position 273 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000007653)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTATTGTGAACGACAGGGAT
Hairpin Sequence:
5'-CCGG-CCTATTGTGAACGACAGGGAT-CTCGAG-ATCCCTGTCGTTCACAATAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTATTGTGAACGACAGGGATCTCGAGATCCCTGTCGTTCACAATAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTATTGTGAACGACAGGGATCTCGAGATCCCTGTCGTTCACAATAGG-3'

Other clones with same target sequence:

(none)