Construct: shRNA TRCN0000007710

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018227.3-514s1c1
DNA Barcode:
CCATTGCAGAAGAAGATCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UBA6 (55236)
Gene Description:
ubiquitin like modifier activating enzyme 6
Transcript:
RefSeq NM_018227.3 (NON-CURRENT)
Match location:
Position 514 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000007710)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATTGCAGAAGAAGATCAAT
Hairpin Sequence:
5'-CCGG-CCATTGCAGAAGAAGATCAAT-CTCGAG-ATTGATCTTCTTCTGCAATGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATTGCAGAAGAAGATCAATCTCGAGATTGATCTTCTTCTGCAATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATTGCAGAAGAAGATCAATCTCGAGATTGATCTTCTTCTGCAATGG-3'

Other clones with same target sequence:

(none)