Construct: shRNA TRCN0000007714
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_018299.1-61s1c1
- DNA Barcode:
- CCTCCTGGAATGACCTTAAAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- UBE2W (55284)
- Gene Description:
- ubiquitin conjugating enzyme E2 W
- Transcript:
- RefSeq NM_018299.1 (NON-CURRENT)
- Match location:
- Position 61 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000007714)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCTCCTGGAATGACCTTAAAT
- Hairpin Sequence:
-
5'-CCGG-CCTCCTGGAATGACCTTAAAT-CTCGAG-ATTTAAGGTCATTCCAGGAGG-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCTCCTGGAATGACCTTAAATCTCGAGATTTAAGGTCATTCCAGGAGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCTCCTGGAATGACCTTAAATCTCGAGATTTAAGGTCATTCCAGGAGG-3'
Other clones with same target sequence:
-
TRCN0000280044
