Construct: shRNA TRCN0000007745

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153371.2-2414s1c1
DNA Barcode:
CCACTAACCTTTGTGAGTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LNX2 (222484)
Gene Description:
ligand of numb-protein X 2
Transcript:
RefSeq NM_153371.2 (NON-CURRENT)
Match location:
Position 2414 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000007745)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTAACCTTTGTGAGTTTA
Hairpin Sequence:
5'-CCGG-CCACTAACCTTTGTGAGTTTA-CTCGAG-TAAACTCACAAAGGTTAGTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTAACCTTTGTGAGTTTACTCGAGTAAACTCACAAAGGTTAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTAACCTTTGTGAGTTTACTCGAGTAAACTCACAAAGGTTAGTGG-3'

Other clones with same target sequence:

(none)