Construct: shRNA TRCN0000007759

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173497.1-669s1c1
DNA Barcode:
GCTGTGTATGATACCTTACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HECTD2 (143279)
Gene Description:
HECT domain E3 ubiquitin protein ligase 2
Transcript:
RefSeq NM_173497.1 (NON-CURRENT)
Match location:
Position 669 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000007759)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTGTATGATACCTTACTT
Hairpin Sequence:
5'-CCGG-GCTGTGTATGATACCTTACTT-CTCGAG-AAGTAAGGTATCATACACAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTGTATGATACCTTACTTCTCGAGAAGTAAGGTATCATACACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTGTATGATACCTTACTTCTCGAGAAGTAAGGTATCATACACAGC-3'

Other clones with same target sequence:

(none)