Construct: shRNA TRCN0000007762

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173497.1-618s1c1
DNA Barcode:
GCCTCATTTAACACCATTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HECTD2 (143279)
Gene Description:
HECT domain E3 ubiquitin protein ligase 2
Transcript:
RefSeq NM_173497.1 (NON-CURRENT)
Match location:
Position 618 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000007762)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCATTTAACACCATTGAA
Hairpin Sequence:
5'-CCGG-GCCTCATTTAACACCATTGAA-CTCGAG-TTCAATGGTGTTAAATGAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCATTTAACACCATTGAACTCGAGTTCAATGGTGTTAAATGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCATTTAACACCATTGAACTCGAGTTCAATGGTGTTAAATGAGGC-3'

Other clones with same target sequence:

(none)