Construct: shRNA TRCN0000007826

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198461.1-531s1c1
DNA Barcode:
GCTGGCTTAAAGAGACAGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LONRF2 (164832)
Gene Description:
LON peptidase N-terminal domain and ring finger 2
Transcript:
RefSeq NM_198461.1 (NON-CURRENT)
Match location:
Position 531 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000007826)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGCTTAAAGAGACAGTTT
Hairpin Sequence:
5'-CCGG-GCTGGCTTAAAGAGACAGTTT-CTCGAG-AAACTGTCTCTTTAAGCCAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGCTTAAAGAGACAGTTTCTCGAGAAACTGTCTCTTTAAGCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGCTTAAAGAGACAGTTTCTCGAGAAACTGTCTCTTTAAGCCAGC-3'

Other clones with same target sequence:

(none)