Construct: shRNA TRCN0000007863

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012199.2-1350s1c1
DNA Barcode:
CCTGATGAAGAATGCCAGCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AGO1 (26523)
Gene Description:
argonaute RISC component 1
Transcript:
RefSeq NM_012199.2 (NON-CURRENT)
Match location:
Position 1350 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000007863)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGATGAAGAATGCCAGCTA
Hairpin Sequence:
5'-CCGG-CCTGATGAAGAATGCCAGCTA-CTCGAG-TAGCTGGCATTCTTCATCAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGATGAAGAATGCCAGCTACTCGAGTAGCTGGCATTCTTCATCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGATGAAGAATGCCAGCTACTCGAGTAGCTGGCATTCTTCATCAGG-3'

Other clones with same target sequence:

(none)