Construct: shRNA TRCN0000007881

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018068.2-3145s1c1
DNA Barcode:
CCAACCCTGTAGAATAAGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIWIL2 (55124)
Gene Description:
piwi like RNA-mediated gene silencing 2
Transcript:
RefSeq NM_018068.2 (NON-CURRENT)
Match location:
Position 3145 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000007881)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAACCCTGTAGAATAAGATT
Hairpin Sequence:
5'-CCGG-CCAACCCTGTAGAATAAGATT-CTCGAG-AATCTTATTCTACAGGGTTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAACCCTGTAGAATAAGATTCTCGAGAATCTTATTCTACAGGGTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAACCCTGTAGAATAAGATTCTCGAGAATCTTATTCTACAGGGTTGG-3'

Other clones with same target sequence:

(none)