Construct: shRNA TRCN0000008053
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_033303.1-1331s1c1
- DNA Barcode:
- CCTGATTTCAAGCCCTCTGAA
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- ADRA1A (148)
- Gene Description:
- adrenoceptor alpha 1A
- Transcript:
- RefSeq NM_033303.1 (NON-CURRENT)
- Match location:
- Position 1331 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this shRNA:
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Sequence Information
- Target Sequence:
- CCTGATTTCAAGCCCTCTGAA
- Hairpin Sequence:
- 5'-CCGG-CCTGATTTCAAGCCCTCTGAA-CTCGAG-TTCAGAGGGCTTGAAATCAGG-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCTGATTTCAAGCCCTCTGAACTCGAGTTCAGAGGGCTTGAAATCAGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCTGATTTCAAGCCCTCTGAACTCGAGTTCAGAGGGCTTGAAATCAGG-3'