Construct: shRNA TRCN0000008057

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000679.2-1093s1c1
DNA Barcode:
CTACCCTTCTTCATCGCTCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADRA1B (147)
Gene Description:
adrenoceptor alpha 1B
Transcript:
RefSeq NM_000679.2 (NON-CURRENT)
Match location:
Position 1093 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000008057)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTACCCTTCTTCATCGCTCTA
Hairpin Sequence:
5'-CCGG-CTACCCTTCTTCATCGCTCTA-CTCGAG-TAGAGCGATGAAGAAGGGTAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACCCTTCTTCATCGCTCTACTCGAGTAGAGCGATGAAGAAGGGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACCCTTCTTCATCGCTCTACTCGAGTAGAGCGATGAAGAAGGGTAG-3'

Other clones with same target sequence:

(none)