Construct: shRNA TRCN0000008117

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001703.1-5109s1c1
DNA Barcode:
GACTGCCCACTGCATATAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADGRB2 (576)
Gene Description:
adhesion G protein-coupled receptor B2
Transcript:
RefSeq NM_001703.1 (NON-CURRENT)
Match location:
Position 5109 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000008117)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTGCCCACTGCATATAAAT
Hairpin Sequence:
5'-CCGG-GACTGCCCACTGCATATAAAT-CTCGAG-ATTTATATGCAGTGGGCAGTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTGCCCACTGCATATAAATCTCGAGATTTATATGCAGTGGGCAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTGCCCACTGCATATAAATCTCGAGATTTATATGCAGTGGGCAGTC-3'

Other clones with same target sequence:

  1. TRCN0000342866