Construct: shRNA TRCN0000008166

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005795.2-2704s1c1
DNA Barcode:
CCTTCCATTTCTACTGTATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CALCRL (10203)
Gene Description:
calcitonin receptor like receptor
Transcript:
RefSeq NM_005795.2 (NON-CURRENT)
Match location:
Position 2704 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000008166)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCCATTTCTACTGTATAA
Hairpin Sequence:
5'-CCGG-CCTTCCATTTCTACTGTATAA-CTCGAG-TTATACAGTAGAAATGGAAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCCATTTCTACTGTATAACTCGAGTTATACAGTAGAAATGGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCCATTTCTACTGTATAACTCGAGTTATACAGTAGAAATGGAAGG-3'

Other clones with same target sequence:

(none)