Construct: shRNA TRCN0000008227

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016557.2-142s1c1
DNA Barcode:
GCACTTATGACTACAGTCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACKR4 (51554)
Gene Description:
atypical chemokine receptor 4
Transcript:
RefSeq NM_016557.2 (NON-CURRENT)
Match location:
Position 142 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000008227)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTTATGACTACAGTCAAT
Hairpin Sequence:
5'-CCGG-GCACTTATGACTACAGTCAAT-CTCGAG-ATTGACTGTAGTCATAAGTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTTATGACTACAGTCAATCTCGAGATTGACTGTAGTCATAAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTTATGACTACAGTCAATCTCGAGATTGACTGTAGTCATAAGTGC-3'

Other clones with same target sequence:

(none)