Construct: shRNA TRCN0000008238

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_078481.1-674s1c1
DNA Barcode:
CCAAACAATACCGTCTGTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADGRE5 (976)
Gene Description:
adhesion G protein-coupled receptor E5
Transcript:
RefSeq NM_078481.1 (NON-CURRENT)
Match location:
Position 674 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000008238)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAACAATACCGTCTGTGAA
Hairpin Sequence:
5'-CCGG-CCAAACAATACCGTCTGTGAA-CTCGAG-TTCACAGACGGTATTGTTTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAACAATACCGTCTGTGAACTCGAGTTCACAGACGGTATTGTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAACAATACCGTCTGTGAACTCGAGTTCACAGACGGTATTGTTTGG-3'

Other clones with same target sequence:

(none)