Construct: shRNA TRCN0000008251

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004767.2-985s1c1
DNA Barcode:
GCCCGCATGTGGTGGTACTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GPR37L1 (9283)
Gene Description:
G protein-coupled receptor 37 like 1
Transcript:
RefSeq NM_004767.2 (NON-CURRENT)
Match location:
Position 985 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000008251)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCGCATGTGGTGGTACTTT
Hairpin Sequence:
5'-CCGG-GCCCGCATGTGGTGGTACTTT-CTCGAG-AAAGTACCACCACATGCGGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCGCATGTGGTGGTACTTTCTCGAGAAAGTACCACCACATGCGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCGCATGTGGTGGTACTTTCTCGAGAAAGTACCACCACATGCGGGC-3'

Other clones with same target sequence:

(none)