Construct: shRNA TRCN0000008253

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_371262.1-1396s1c1
DNA Barcode:
CCCACATTATATGAACTTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADGRL4 (64123)
Gene Description:
adhesion G protein-coupled receptor L4
Transcript:
RefSeq XM_371262.1 (NON-CURRENT)
Match location:
Position 1396 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000008253)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCACATTATATGAACTTGAA
Hairpin Sequence:
5'-CCGG-CCCACATTATATGAACTTGAA-CTCGAG-TTCAAGTTCATATAATGTGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCACATTATATGAACTTGAACTCGAGTTCAAGTTCATATAATGTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCACATTATATGAACTTGAACTCGAGTTCAAGTTCATATAATGTGGG-3'

Other clones with same target sequence:

(none)