Construct: shRNA TRCN0000008280

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153024.1-997s1c1
DNA Barcode:
CCGTTTCAACGTCTCAACCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OR7E5P (219445)
Gene Description:
olfactory receptor family 7 subfamily E member 5 pseudogene
Transcript:
RefSeq NM_153024.1 (NON-CURRENT)
Match location:
Position 997 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000008280)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTTTCAACGTCTCAACCTT
Hairpin Sequence:
5'-CCGG-CCGTTTCAACGTCTCAACCTT-CTCGAG-AAGGTTGAGACGTTGAAACGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTTTCAACGTCTCAACCTTCTCGAGAAGGTTGAGACGTTGAAACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTTTCAACGTCTCAACCTTCTCGAGAAGGTTGAGACGTTGAAACGG-3'

Other clones with same target sequence:

(none)